multipotential leukemia line k562  (DSMZ)

Journal: International journal of cancer

Article Title: Mesenchymal-epithelial transitions: spontaneous and cumulative syntheses of epithelial marker molecules and their assemblies to novel cell junctions connecting human hematopoietic tumor cells to carcinomatoid tissue structures.

doi: 10.1002/ijc.26227

Figure Lengend Snippet: Figure 2. Identification and localization of desmosomal proteins in hematopoietic K562 cells in clusters of adhering junctions and half-junctions

Article Snippet: Cell cultures and special treatments Cells of the human multipotential leukemia line K562 were repeatedly obtained from the American Type Culture Collection or the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany) and usually grown in Iscove’s Modified Dulbeccos’s Medium (Invitrogen, Karlsruhe, Germany), supplemented with 10% fetal calf serum and 2 mM glutamine, in suspension culture, in small plastic cylinders, or as cells allowed to adhere to a substratum (plastic surface, slides or coverslips, without or with coating using poly-L-lysine, collagen or fibronectin).

Techniques:

Journal: International journal of cancer

Article Title: Mesenchymal-epithelial transitions: spontaneous and cumulative syntheses of epithelial marker molecules and their assemblies to novel cell junctions connecting human hematopoietic tumor cells to carcinomatoid tissue structures.

doi: 10.1002/ijc.26227

Figure Lengend Snippet: Figure 2. Identification and localization of desmosomal proteins in hematopoietic K562 cells in clusters of adhering junctions and half-junctions

Article Snippet: Cell cultures and special treatments Cells of the human multipotential leukemia line K562 were repeatedly obtained from the American Type Culture Collection or the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany) and usually grown in Iscove’s Modified Dulbeccos’s Medium (Invitrogen, Karlsruhe, Germany), supplemented with 10% fetal calf serum and 2 mM glutamine, in suspension culture, in small plastic cylinders, or as cells allowed to adhere to a substratum (plastic surface, slides or coverslips, without or with coating using poly-L-lysine, collagen or fibronectin).

Techniques: